@InProceedings{WacheskHuCoTaVaCoTr:2020:SyChDi,
               author = "Wachesk, Cristiane da Costa and Hurtado, Carolina Ramos and 
                         Correia, Rebeca Falc{\~a}o Borja de Oliveira and Tada, Dayane 
                         Batista and Vasconcelos, Get{\'u}lio and Corat, Evaldo Jos{\'e} 
                         and Trava-Airoldi, Vladimir Jesus",
          affiliation = "{Universidade Federal de S{\~a}o Paulo (UNIFESP)} and 
                         {Universidade Federal de S{\~a}o Paulo (UNIFESP)} and 
                         {Universidade Federal de S{\~a}o Paulo (UNIFESP)} and 
                         {Universidade Federal de S{\~a}o Paulo (UNIFESP)} and {Instituto 
                         de Estudos Avan{\c{c}}ados (IEAv)} and {Instituto Nacional de 
                         Pesquisas Espaciais (INPE)} and {Instituto Nacional de Pesquisas 
                         Espaciais (INPE)}",
                title = "Synthesis and characterization of diamond nanoparticles with 
                         methylene blue for cancer photodynamic therapy",
                 year = "2020",
         organization = "Encontro de Outono Sociedade Brasileira de F{\'{\i}}sica",
             keywords = "HFCVD diamond, PDT, CVD Diamond Nanoparticles (CVD-DNPs), Drug 
                         Delivery, Melanoma.",
             abstract = "Diamond nanoparticles (DNPs) have showed in vitro and in vivo 
                         biomedical applicability due to their low toxicity and 
                         biocompatibility. Recent studies have focused on the potential use 
                         of (DNPs) as suitable vehicles for improving drug delivery in 
                         cancer treatment. The advantages of DNPs lies in their high 
                         stability and small size compared to other carbon-based 
                         nanomaterials. In this work, CVD-diamond nanoparticles (CVD-DNPs) 
                         were synthesized and evaluated regarding their application as a 
                         new drug delivery platform for metastatic melanoma therapy. A new 
                         synthesis technique developed DNPs from CVD diamond thin film. 
                         This type of diamond has the same physical and chemical properties 
                         as a natural diamond: extreme hardness, excellent thermal 
                         conductivity, low coefficient of friction, biocompatibility, 
                         chemically inert for temperatures below 8000 C, among others. The 
                         main objective of this study was to produce CVD-DNPs by laser 
                         ablation and to evaluate their cytotoxicity. A pulsed, 
                         ytterbium-doped fiber (Yb) was used to form DNPs in the pure 
                         aqueous medium (Milli-Q). The characterization by FT-IR, X-Ray, 
                         DLS, RAMAN, SEM, and TEM demonstrated the successful synthesis of 
                         CVD-DNPs with a hydrodynamic diameter of 57 and 54 nm. In vitro 
                         studies performed for 24h and 48h resulted in viability of 70-80% 
                         of cells incubated with CVD-DNPs at 250 \μg/mL, which 
                         demonstrated a insignificant cytotoxic effect. The irradiation was 
                         performed with laser light with \λ = 665nm (0,3J/cm2), for 1 
                         min. Quantification of cell viability was performed by the 
                         colorimetric method, and performed a reading in a microplate 
                         reader at 540nm. Determination of Methylene Blue was AM (1,88 
                         \μg / mL), (2,82 \μg / mL), (5,64 \μg / mL) 
                         respectively, in the presence and absence of light. Using the 
                         protocol of PDT observed that there is one of the cytotoxic 
                         effects of AM with statistical significance. The results showed 
                         that both platforms promoted the solubility of (MB / water) and 
                         (MB / CVD-DNPs) which is essential in biological applications. MB 
                         conjugated with CVD-DNPs proved to be an adequate platform for the 
                         application of PDT. Thus, these results suggest a potential use of 
                         CVD-DNPs as a drug delivery platform for antitumoral therapy.",
  conference-location = "Online",
      conference-year = "23 a 26 nov.",
        urlaccessdate = "28 abr. 2024"
}